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After TBST washing (three times, 5 min per wash), the membranes were incubated with the indicated primary antibodies at 4 °C overnight with shaking

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An 8-week randomized double-blind trial comparing GHK-Cu in nano-lipid carrier against Matrixyl 3000 showed 55.8% reduction in wrinkle volume versus control and 31.6% reduction versus the active comparator